Human adenoviruses belonging to the family Adenoviridae, family Mastadenovirus. Adenoviruses are medium size, double-stranded DNA icosahedral viruses without an envelope. Adenoviruses can cause respiratory, ocular or gastrointestinal disorders, especially occurring in children.

Adenovirus infections are common, widespread globally and occur throughout the year. Adenoviruses have been recently investigated as pathogens with a high morbidity and mortality in immunocompromised patients. Clinical signs include pneumonia, hepatitis, haemorrhagic cystitis, colitis, pancreatitis, meningoencephalitis and disseminated disease according to the attacked organ and the patient's age and serotype.

If there is the suspician of an adenovirus disease, blood samples should be tested with PCR detection because adenoviral DNA from blood has a predictive value for disease prevalence. Monitoring blood samples is a common practice in recipients of bone marrow transplant, particularly in the pediatric population.

Examination

Amplification and detection of the viral genome by real-time PCR is a highly sensitive examination, which is particularly useful when a noninfectious virus is present or if there is a need for a fast result. The kit still detects all known serotypes of human adenoviruses.

  • Method:
    Quantitative detection by real-time PCR of all known adenovirus serotypes. The sensitivity of detection of 10 copies / reaction.
  • Material:
    • whole blood (EDTA tube - transport to the laboratory within 24 hours from extracting it in room temperature)
    • plasma (after centrifugation of whole blood in EDTA tube at 1200xg for 10 min. to 7 days may be stored at 2/8 °C, longer at -18 / -22 °C)
    • cerebrospinal fluid, urine, biopsy (-18 / -22 °C) (from * labeled material are advisable to carry out only qualitative detection)

References

  1. Marcela Echavarría: Adenoviruses in Immunocompromised Hosts, Clin Microbiol Rev. 2008 October; 21(4): 704–715.
  2. B. Ghebremedhin: Human adenovirus: Viral pathogen with increasing importance : Eur J Microbiol Immunol (Bp) 2014 March; 4(1): 26–33.
  3. Votava M. a kol.: Lékařská mikrobiologie speciální, Neptun, 2003.